Biolegend, 406204, Biotin anti-mouse IgM, 500μg

This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-C b and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-C e can be detected with this antibody.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality testedELISA Detection - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.
Application References(PubMed link indicates BioLegend citation): Stall AM, et al. 1984. J. Immunol. 132:787. Velardi A, et al. 1984. J. Immunol. 133:2098.
Product Citations: Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Sundling C, et al. 2021. Immunity. 54(5):988-1001.e5. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed
RRID: AB_315037 (BioLegend Cat. No. 406204)
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgMb on UniProt.org
Clone: AF6-78
Regulatory Status: RUO
Other Names: Igh-6b
Isotype: Mouse (BALB/c) IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.