Product Description
This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
500microl
Verified Reactivity: Rabbit
Antibody Type: Polyclonal
Host Species: Donkey
Formulation: Aqueous buffered solution containing a carrier protein and preservatives.
Preparation: The polyclonal antibody was purified from donkey antiserum by rabbit immunoglobulin affinity chromatography.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expirations date of your lot of product
Application: WB - Quality tested ELISA, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
Product Citations: Hunt D, et al. 2018. PLoS One. 13:e0200764. PubMed Apte S, et al. 2023. Sci Adv. 9:eade1851. PubMed Evans JG, et al. 2023. Viruses. 15:. PubMed Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed Shukla S, et al. 2022. FASEB J. 36: . PubMed Wang L, et al. 2022. Viruses. 14: . PubMed Biering SB, et al. 2022. Nat Commun. 13:7630. PubMed Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed Zanuttigh E, et al. 2023. Pharmaceutics. 15: . PubMed Miki H, et al. 2020. J Immunol. 204:753. PubMed Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed Nitiss JL, et al. 2021. Curr Protoc. 1:e250. PubMed Adkar-Purushothama C, et al. 2015. Plant Cell. 27: 2178-2194. PubMed Zech M, et al. 2018. Cold Spring Harb Mol Case Stud. 4:. PubMed Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed Lam DD, et al. 2022. J Neurosci. 42:1557. PubMed Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed Xu H, et al. 2021. Microb Cell Fact. 20:115. PubMed Wang Y, et al. 2016. Cell Death Dis. 7:e2458. PubMed Lee K, et al. 2020. Biology (Basel). 9:00. PubMed Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed Ohta E, et al. 2011. FEBS Lett. 585:2165. PubMed Saiz ML, et al. 2021. Clin Epigenetics. 13:187. PubMed Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed Ashley CL, et al. 2019. Viruses. 11:3. PubMed Chan TC, et al. 2021. Clin Transl Med. 11:e674. PubMed Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed Kauke MJ, et al. 2018. Mol Cancer Ther. 1.939583333. PubMed Kiianitsa K, et al. 2020. DNA Repair (Amst). 96:102977. PubMed Moffat J, et al. 2013. Vaccine. 31:2310. PubMed Yu C, et al. 2021. Front Genet. 12:689902. PubMed Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed Kiianitsa K, et al. 2021. J Leukoc Biol. 110:829. PubMed Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed Veronica Margarecaesha Anyndita N, et al. 2018. Heliyon. 4:e00564. PubMed Luis-Garcia ER, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID: AB_2099368 (BioLegend Cat. No. 406401)
Gene ID: 100343867
UniProt: View information about Rabbit IgG on UniProt.org
Clone: Poly4064
Regulatory Status: RUO
Isotype: Donkey Polyclonal Ig
Order Guidelines
1. Price & Stock Available on Request. Click to send email to: service@iright.com
2. Please DO NOT make payment before confirmation.
3. Minimum order value of $1,000 USD required.
Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924