Biolegend, 406412, Alexa Fluor® 555 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
100μg
Verified Reactivity: Rabbit
Antibody Type: Polyclonal
Host Species: Donkey
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
Product Citations: Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed Chae SA, et al. 2023. J Physiol. 601:2371. PubMed Koop K, et al. 2023. Front Immunol. 14:1163198. PubMed Tayoun T, et al. 2022. JCI Insight. 7: . PubMed Frigon ÉM, et al. 2022. Front Neuroanat. 16:957358. PubMed Brown CW, et al. 2020. Developmental Cell. 51(5):575-586.e4.. PubMed Son JS, et al. 2020. Cell Rep. 33:108461. PubMed Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed Divito SJ, et al. 2020. J Clin Invest. 130:4624. PubMed Kissane RWP, et al. 2021. Journal of Anatomy. . PubMed Baba T, et al. 2020. eLife. 9:e54257.. PubMed
RRID: AB_2563181 (BioLegend Cat. No. 406412)
Gene ID: 100343867
UniProt: View information about Rabbit IgG on UniProt.org
Clone: Poly4064
Regulatory Status: RUO
Isotype: Donkey Polyclonal Ig