Biolegend, 406503, Biotin anti-mouse IgM Antibody, 50μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality testedELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Lai M, et al. 2016. Nat Commun. 7:12207. PubMed Gonzalez-Martin A, et al. 2016. Nat Immunol. 17:433-440. PubMed Zhao M, et al. 2019. Cell Rep. 26:652. PubMed Suzuki M, et al. 2021. Sci Adv. 7:. PubMed Dong S, et al. 2021. Nature. 591:117. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed Boulianne B, et al. 2013. J Immunol. 191:4521. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed Yamada H, et al. 2018. J Immunol. 201:3492. PubMed
RRID: AB_315053 (BioLegend Cat. No. 406503) AB_315054 (BioLegend Cat. No. 406504)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.