Biolegend, 406515, APC/Cyanine7 anti-mouse IgM Antibody, 25μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Tan J, et al. 2021. iScience. 24(8):102835. PubMed Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed Choi JY, et al. 2020. Proc Natl Acad Sci U S A. 117:6042. PubMed González-Hernández A, et al. 2016. Sci Rep. 6:29496. PubMed Meulder F, et al. 2015. Int J Parasit. 45: 637-646. PubMed Pighi C, et al. 2021. Blood Adv. 5:5239. PubMed Weber G, et al. 2014. J Exp Med. 211:1243. PubMed Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed Singh M, et al. 2020. Cell. 180(5):878-894. PubMed Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed González-Hernández A, et al. 2017. Int J Parasitol. 10.1016/j.ijpara.2017.07.002. PubMed Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Grit G, et al. 2014. Vet Parasitol. 202:145. PubMed
RRID: AB_10690815 (BioLegend Cat. No. 406515) AB_10660305 (BioLegend Cat. No. 406516)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ