Biolegend, 406704, Biotin anti-mouse IgG2b Antibody, 500μg

The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality testedELISA
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Heyburn L, et al. 2016. J Neurochem. 610:139. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Sellars M, et al. 2009. J Exp Med. 206:1073. PubMed Esposito L, et al. 2014. J Immunol. 193:889. PubMed Sundling C, et al. 2021. Immunity. 54(5):988-1001.e5. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
RRID: AB_315066 (BioLegend Cat. No. 406703) AB_315067 (BioLegend Cat. No. 406704)
Gene ID: 16016
UniProt: View information about IgG2b on UniProt.org
Clone: RMG2b-1
Regulatory Status: RUO
Other Names: Immunoglobulin G2b
Isotype: Rat IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.