Biolegend, 407105, FITC anti-mouse IgG2a Antibody, 50μg

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Gavin AL, et al.2006. Science 314:1936. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
Product Citations: Liang Q, et al. 2022. iScience. 25:104043. PubMed Fuchs D, et al. 2010. Biochem Biophys Res Commun. 394:1098. PubMed Gradiz R, et al. 2016. Sci Rep. 6:21648. PubMed East J, et al. 2012. J Vis Exp. 70: 4333. PubMed Lin Y, et al. 2011. Transpl Immunol. 25:194. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Pandya P, et al. 2020. PLoS One. 15:e0233961. PubMed Cronin J, et al. 2016. Mucosal Immunol. 10.1038/mi.2015.131. PubMed
RRID: AB_493294 (BioLegend Cat. No. 407105) AB_493295 (BioLegend Cat. No. 407106)
Gene ID: 668478
UniProt: View information about IgG2a on UniProt.org
Clone: RMG2a-62
Regulatory Status: RUO
Other Names: Immunoglobulin G2a
Isotype: Rat IgG
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.