Biolegend, 407111, PerCP/Cyanine5.5 anti-mouse IgG2a Antibody, 25μg

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Gavin AL, et al.2006. Science 314:1936. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
Product Citations: Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Hayashi Y, et al. 2017. J Biol Chem. 292(3):1122-1141. PubMed
RRID: AB_2562007 (BioLegend Cat. No. 407111) AB_2562008 (BioLegend Cat. No. 407112)
Gene ID: 668478
UniProt: View information about IgG2a on UniProt.org
Clone: RMG2a-62
Regulatory Status: RUO
Other Names: Immunoglobulin G2a
Isotype: Rat IgG
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.