Biolegend, 407511, Alexa Fluor® 647 anti-rat IgG2a Antibody, 25μg

The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
25μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mixed rat Igs
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Inoue S, et al. 2006. Cancer Research 66:7741.
Product Citations: Peymanfar Y, et al. 2023. Int J Mol Sci. 24:. PubMed Geng S, et al. 2024. iScience. 110097:27. PubMed Porsche CE, et al. 2021. JCI Insight. 6:. PubMed Magod P, et al. 2021. Cell Reports. 36(5):109480. PubMed Park JY, et al. 2019. Theranostics. 9:3398. PubMed
RRID: AB_2716139 (BioLegend Cat. No. 407511) AB_2716140 (BioLegend Cat. No. 407512)
Gene ID: 367586
UniProt: View information about IgG2a on UniProt.org
Clone: MRG2a-83
Regulatory Status: RUO
Other Names: Immunoglobulin G2a
Isotype: Mouse IgG