Biolegend, 408108, APC anti-phycoerythrin (PE) Antibody, 100μg

The PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cyanine5, PE/Cyanine5.5 and PE/Cyanine7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Phycoerythrin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Taylor RT, et al. 2007. J. Immunol. 178:5659.
Product Citations: Greenshields-Watson A, et al. 2020. Cell Rep. 32:107885. PubMed
RRID: AB_2814387 (BioLegend Cat. No. 408107) AB_2814388 (BioLegend Cat. No. 408108)
Gene ID: NA
UniProt: View information about Phycoerythrin on UniProt.org
Clone: PE001
Regulatory Status: RUO
Other Names: PE
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.