Biolegend, 408604, Biotin anti-mouse IgM, 500μg

This antibody was raised by immunization with IgM a mAb TC31. It specifically reacts with mouse IgM of Igh-C a and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality testedIHC, ELISA Detection - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Sieckmann D, et al. 1991. Hybridoma 10:121. Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.
Product Citations: Li Y, et al. 2012. Blood. 3436:120. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed
RRID: AB_940535 (BioLegend Cat. No. 408603) AB_940537 (BioLegend Cat. No. 408604)
Gene ID: 16019
UniProt: View information about IgMa on UniProt.org
Clone: MA-69
Regulatory Status: RUO
Other Names: Igh-6a
Isotype: Mouse (C57BL/6) IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.