Biolegend, 408609, PE/Cyanine7 anti-mouse IgM, 25μg

This antibody was raised by immunization with IgM a mAb TC31. It specifically reacts with mouse IgM of Igh-C a and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Sieckmann D, et al. 1991. Hybridoma 10:121. Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.
Product Citations: Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
RRID: AB_2728433 (BioLegend Cat. No. 408609) AB_2728434 (BioLegend Cat. No. 408610)
Gene ID: 16019
UniProt: View information about IgMa on UniProt.org
Clone: MA-69
Regulatory Status: RUO
Other Names: Igh-6a
Isotype: Mouse (C57BL/6) IgG1, κ