Biolegend, 410501, Purified anti-Streptavidin Antibody, 500μg

Streptavidin is a 52 kD tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.
500μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Streptavidin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 3A20.2 purified antibody is useful as a third step reagent to amplify dim or low signals in many applications. This antibody does not cross-react with avidin.
Product Citations: Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed Andrechak JC, et al. 2022. Cancers (Basel). 14:. PubMed Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed Jiang L, et al. 2018. J Exp Med. 215:1337. PubMed Bartsch YC, et al. 2018. Front Immunol. 1.196527778. PubMed Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed Telford W, et al. 2017. Cytometry A. 91:314. PubMed Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed
RRID: AB_2564569 (BioLegend Cat. No. 410501)
Structure: 52 kD tetrameric protein.
Function: Streptavidin binds to biotin with high affinity.
Ligand/Receptor: Biotin
Antigen References: 1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343. 2. Zhao X, et al. 2013. J. Anal Methods Chem. 58:1093. 3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135. 4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113. 5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.
Gene ID: NA
UniProt: View information about Streptavidin on UniProt.org
Clone: 3A20.2
Regulatory Status: RUO
Other Names: SAv
Isotype: Mouse IgG2b, κ