Biolegend, 410503, PE anti-Streptavidin Antibody, 25μg

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.
25μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Streptavidin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 3A20.2 purified antibody is useful as a third step reagent to amplify dim or low signals in many applications. This antibody does not cross-react with avidin.
Product Citations: Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed Knyphausen P, et al. 2023. Nat Commun. 14:768. PubMed Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed Silvestre-Roig C, et al. 2019. Nature. 569:236. PubMed Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed Iwabuchi R, et al. 2018. Front Immunol. 9:1042. PubMed Niitsuma S, et al. 2020. Int Immunol. 283:32. PubMed Shi H, et al. 2020. Immunity. 51(6):1012-1027.e7.. PubMed Oberhardt V, et al. 2021. Nature. 597:268. PubMed Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed Wells AC et al. 2017. eLife. 6 pii: e26398. PubMed Shen B, et al. 2019. Elife. 8:e42274. PubMed Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
RRID: AB_2571914 (BioLegend Cat. No. 410503) AB_2571915 (BioLegend Cat. No. 410504)
Structure: 52.8 kD tetrameric protein.
Function: Streptavidin binds to biotin with high affinity.
Ligand/Receptor: Biotin
Antigen References: 1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343. 2. Zhao X, et al. 2013. J. Anal Methods Chem. 58:1093. 3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135. 4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113. 5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.
Gene ID: NA
UniProt: View information about Streptavidin on UniProt.org
Clone: 3A20.2
Regulatory Status: RUO
Other Names: SAv
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.