Biolegend, 421501, TMB High Sensitivity Substrate Solution, 60ml

The TMB High Sensitivity (TMB HS) Substrate solution is an one-step working solution for HRP (horse-radish peroxidase) labeled conjugates. TMB HS substrate solution provides high signal and high sensitivity for ELISA. It is recommended for cytokine ELISA reagent sets. The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H 2 SO 4 or 1M H 3 PO 4 (resulting in a yellow color), and read at 450 nm.
60ml
Storage & Handling: The TMB High Sensitivity Substrate Solution should be stored between 2°C and 8°C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Zeng S, et al. 2008. Int Immunopharmacol. 10:1707. PubMed Eisenach PA, et al. 2010. J. Cell Sci. 123:4182. PubMed Fan YY, et al. 2012. J Lipid Res. 53:1287. PubMed Querol L, et al. 2014. Neurology. 82:879. PubMed Kiianitsa K, et al. 2104. Nucleic Acids Res. 42:108. PubMed Nogales A, et al. 2014. J Virol. 88:10525. PubMed
Product Citations: Querol L, et al. 2014. Neurology. 82:879. PubMed Fan Y, et al. 2012. J Lipid Res. 53:1287. PubMed Cho M, et al. 2016. J Immunol. 197: 1065 - 1073. PubMed Nogales A, et al. 2017. J Virol. 10.1128/JVI.00720-17. PubMed Zeng S, et al. 2008. Int Immunopharmacol. 10:1707. PubMed Sheppard S, et al. 2017. Nat Commun. 8:13930. PubMed Maizels K 2014. Nucleic Acids Res. 42:108. PubMed Eisenach P, et al. 2010. J Cell Sci. 123:4182. PubMed Sinha D, et al. 2020. Anal Biochem. 113827:608. PubMed Nogales A, et al. 2014. J Virol. 88:10525. PubMed Rother N, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01136. PubMed
Gene ID: NA
Regulatory Status: RUO
Other Names: TMB HS Substrate
Q: What is the difference between your two substrate solutions: TMB Substrate Reagent Set (Cat. No. 421101) and TMB High Sensitivity Substrate Solution (Cat. No. 421501)?
A: TMB Substrate Reagent Set (Cat. No. 421101) contains two components, which need to be mixed immediately prior to use. The TMB High Sensitivity Substrate Solution (Cat. No. 421501) contains everything in one solution with special stabilizers. It is a high kinetic substrate solution which generally gives higher signal. The type of substrate used for an assay can affect the optical density (OD) achievable and the time required to achieve the desired OD.