Biolegend, 425101, Tag-it Violet™ Proliferation and Cell Tracking Dye, 1kit

Tag-it Violet™ Proliferation and Cell Tracking Dye can be used for cell tracking and for proliferation assays. Tag-it Violet™ passively diffuses into the cell where esterases cleave acetoxymethyl esters (AM) on the molecule, Tag-it Violet™ then covalently attaches to intracellular proteins enabling its long-term retention. Tag-it Violet™ is excited with a fluorescence wavelength of 395 nm, and emits at 455 nm. Cells labeled wtih Tag-it Violet™ can be analyzed by flow cytometry in instruments equipped with a violet laser, or visualized by fluorescence microscopy using a DAPI filter.
1kit
Preparation: The Tag-it Violet™ Proliferation and Cell Tracking Dye is composed of lyophilized Tag-it Violet™ and anhydrous DMSO. For reconstitution, bring the kit to room temperature, add 50 µl of DMSO to one vial of Tag-it Violet™ dye until fully dissolved.
Storage & Handling: The Tag-it Violet™ Proliferation and Cell Tracking Dye should be stored at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the Tag-it Violet™, use immediately, or store at -20°C in a dry place and protected ifrom light, preferably in a dessicator or in a container with dessicant for no more than one month.
Application: FC - Quality tested ICC - Verified
Recommended Usage: This lot has been tested by flow cytometry for in vitro cell proliferation. It can be used at concentrations ranging from 1 - 20 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.
Application Notes: The molecular weight of Tag-it Violet™ Proliferation and Cell Tracking Dye is 489 kD. The maximum excitation and emission wavelengths of Tag-it Violet™ Proliferation and Cell Tracking Dye are 395 nm and 455 nm, respectively. Each 122.25 µg of Tag-it Violet™ Proliferation and Cell Tracking Dye may be reconstituted with 50 µl of anhydrous DMSO to yield a stock concentration of 5 mM. Materials Provided: 5 vials x 122.25 µg Tag-it Violet™ 500 µl anhydrous DMSO Tag it-Violet™ Labeling Procedure: 1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial. 2. Prepare stock solution by reconstituting 1 vial of lyophilized Tag-it Violet™ dye in 50 µl of anhydrous DMSO to make a 5 mM solution. 3. Prepare a 5 µM working solution by diluting 1 µL of 5 mM Tag-it Violet™ stock solution in 1 mL PBS for every 1 mL of cell suspension (or at an optimal working concentration as determined by titration). 4. Spin down and resuspend cells at 10-100 x 106 cells/mL in the Tag-it Violet™ working solution. 5. Incubate cells for 20 minutes at room temperature or at 37°C, and keep protected from light. 6. Quench the staining by adding five times the original staining volume of cell culture medium containing 10% FBS. 7. Pellet cells and resuspend in pre-warmed cell culture medium. 8. Incubate cells for 10 minutes. 9. After incubation, Tag-it Violet™ labeled cells are ready for downstream applications or analysis.
Product Citations: Mehus AA, et al. 2023. Int J Mol Sci. 24:. PubMed Alsavaf MB, et al. 2022. Br J Haematol. 197:e88. PubMed Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed Zaiatz-Bittencourt V, et al. 2023. Sci Rep. 13:2715. PubMed Sheng YR, et al. 2022. Cell Mol Life Sci. 79:173. PubMed Olivieri D, et al. 2021. EMBO J. 40:e106818. PubMed Santelli A, et al. 2019. J Am Heart Assoc. 8:e012584. PubMed Littwitz-Salomon E, et al. 2021. Nat Commun. 12:5376. PubMed Denham EM, et al. 2019. PLoS Biol. 17:e3000549. PubMed Kumar V, et al. 2022. JACC Basic Transl Sci. 7:1038. PubMed Guo X, et al. 2021. iScience. 24:103295. PubMed
Structure: 489 kD.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience
Gene ID: NA
Regulatory Status: RUO
Other Names: Tag-It Violet Proliferation Cell Tracking Dye