Biolegend, 427504, Rapamycin, 1kit

Rapamycin is an antifungal metabolite produced by Streptomyces hygroscopicus . Rapamycin is a potent and specific mTOR inhibitor. This allosteric inhibitor of mammalian mTORC1 induces autophagy and also acts as an immunosuppressant. mTORC1 is a central integrator of nutrient signaling pathways and stimulates cell growth and proliferation by activating anabolic processes inhibiting catabolic process such as autophagy.
1kit
Formulation: 1 vial of Rapamycin (10mg, lyophilized), 1 vial of DMSO
Preparation: Solubility: DMSO: 18.28 mg/mL (20 mM) Preparation of stock solution: Resuspend 10 mg of lyophilized Rapamycin in 546.9 µL of fresh DMSO to make a 20 mM stock solution
Storage & Handling: Store lyophilized powder at -20°C. Protect from light. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency.
Application: Cell Culture - Verified
Recommended Usage: Rapamycin was used for induction of Autophagy in a range from 1 to 40 µM. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
Function: mTOR inhibitor, induced autophagy, immunosuppressant
Antigen References: Ballou, et al. 2008. J Chem Biol. 1:27. Fleming, et al. 2011. Nat Chem Biol. 7:9. Galluzzi, et al. 2017. Nat Rev Drug Discov. 16:487.
Gene ID: NA
Regulatory Status: RUO
Other Names: Autophagy inducer, mTOR inhibitor