Biolegend, 430907, LEGEND MAX™ Mouse TNF-α ELISA Kit, 1precoatedplate

TNF-a is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-a. TNF-a forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-a has also been described. TNF-a binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
1precoatedplate
Verified Reactivity: Mouse
Application: ELISA
Product Citations: McKnight Q, et al. 2020. J Bone Miner Res. 35:1352. PubMed Wiesenthal AA, et al. 2023. Biology (Basel). 12:. PubMed Sun Q, et al. 2023. Transl Neurosci. 14:20220273. PubMed Lliberós C, et al. 2021. Front Cell Dev Biol. 8:628473. PubMed Hu CH, et al. 2022. Small Methods. 6:e2100763. PubMed Yokozeki Y, et al. 2021. BMC Musculoskelet Disord. 22:634. PubMed Ma D, et al. 2021. Sci Adv. :7. PubMed Pan W, et al. 2020. Exp Ther Med. 19:1695. PubMed Du L, et al. 2022. Aging (Albany NY). 14:6626. PubMed Minati MA, et al. 2021. Antioxidants (Basel). 10:. PubMed Yin Y, et al. 2022. Front Pharmacol. 13:882924. PubMed Tao W, et al. 2020. Sci Transl Med. 12: . PubMed Wu D, et al. 2022. Cell Death Dis. 13:731. PubMed Wu H, et al. 2019. Autophagy. :1. PubMed Zhang P, et al. 2022. BMC Med. 20:435. PubMed Chang LC, et al. 2020. Biomedicines. 8:00. PubMed Xu X, et al. 2018. Cell. 175:1336. PubMed Lian H, et al. 2021. Nat Microbiol. 6:658. PubMed Xiang K, et al. 2022. Front Immunol. 13:814319. PubMed Tian M, et al. 2020. Cell Rep. 108297:33. PubMed Chen H, et al. 2021. Invest Ophthalmol Vis Sci. 62:11. PubMed Kueanjinda P, Palaga S 2015. Sci Rep. 5: 12784. PubMed Yu WQ, et al. 2019. J Zhejiang Univ Sci B. 20:355. PubMed Tung YT, et al. 2021. Antioxidants (Basel). 10:. PubMed Lu Q, et al. 2022. Biomed Res Int. 2022:9427076. PubMed Lei G, et al. 2017. Antimicrob Agents Chemother. 10.1128/AAC.01226-17. PubMed Chang C, et al. 2021. Mol Med Rep. :23. PubMed Su L, et al. 2019. EMBO J. 38:. PubMed Wang X, et al. 2022. Front Cell Infect Microbiol. 12:839592. PubMed Zhang SJ, et al. 2021. Adv Sci (Weinh). 8:2003410. PubMed Huang WC, et al. 2022. Sci Rep. 12:3612. PubMed
Sensitivity: 1.5 pg/ml
Standard Range: 15.6-1,000 pg/mL
Cell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 21926
UniProt: View information about TNF-alpha on UniProt.org
Regulatory Status: RUO
Other Names: TNF-a ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.