Biolegend, 431508, LEGEND MAX™ Human IL-8 ELISA Kit, 5precoatedplates

Human IL-8 (CXCL8, neutrophil chemotactic factor, neutrophil activating protein, monocytes derived neutrophil chemotactic factor) is a member of the alpha (C-X-C) subfamily of chemokines. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation leading to local inflammation in damaged or infected tissues. BioLegend’s LEGEND MAX™ Human IL-8 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-8 specific monoclonal antibody is pre-coated on a 96-well strip-well plate. BioLegend’s LEGEND MAX™ Human IL-8 ELISA Kit is specifically designed for the accurate quantitation of Human IL-8 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Shi JX, et al. 2012. Am J Physiol Cell Mol Physiol. 302:793. PubMed Kummaraputugu AB, et al. 2013. J. Biol Chem. 288:4681. PubMed Shi JX, et al. 2014. Cell Signal. 26:2390. PubMed
Product Citations: Shishkova D, et al. 2022. Int J Mol Sci. 23: . PubMed Shi J, et al. 2014. Int Immunopharmacol. 22:480. PubMed Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed Zhang Y, et al. 2018. Cell Mol Gastroenterol Hepatol. 5:611. PubMed Shi J, et al. 2014. Cell Signal. 26:2390. PubMed Spittaels KJ, et al. 2021. iScience. 24:102575. PubMed Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed Shi J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L793. PubMed Shi JX, et al. 2018. Mol Med Rep. 2.361805556. PubMed Kummarapurugu A, et al. 2013. J Biol Chem. 288:4861. PubMed Dudau M, et al. 2021. Front Pharmacol. 12:737571. PubMed
Sensitivity: 4.4 pg/mL
Standard Range: 15.6-1,000 pg/mL
Cell Sources: Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 3576
UniProt: View information about IL-8 on UniProt.org
Regulatory Status: RUO
Other Names: IL-8 Pre-coated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.