Biolegend, 433107, LEGEND MAX™ Human/Mouse/Rat Phospho-Tau (Thr217) ELISA Kit, 1precoatedplate

Tau is a 441-amino acid protein that binds along microtubules in order to stabilize them. Phosphorylation of Tau reduces its affinity for microtubules, leading to microtubule destabilization. Hyperphosphorylated Tau can assemble into higher order structures, including oligomers and neurofibrillary tangles (NFT). NFTs are a hallmark of several neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, and frontotemporal dementia. The level of phosphorylated Tau, therefore, is an important biomarker for the progression of these diseases. Phosphorylation of Tau at threonine 217 has become an important marker in Alzheimer's disease, for example, because it can be detected years ahead of typical disease symptoms and correlates well with disease severity. The BioLegend LEGEND MAX™ Human/Mouse/Rat Phospho-Tau (Thr217) ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with mouse monoclonal Tau antibody. The detection antibody is a biotinylated rabbit polyclonal phospho-Tau (Thr217) antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. This kit is specifically designed for the accurate quantitation of phospho-Tau (Thr217) in human/mouse/rat brain lysates. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human, Mouse, Rat
Application: ELISA
Sensitivity: 8.63 ± 1.71 pg/mL
Standard Range: 47 – 3000 pg/mL
Molecular Family: Tau
Gene ID: 4137
UniProt: View information about Phospho-Tau Thr217 on UniProt.org
Regulatory Status: RUO
Other Names: Microtubule-Associated Protein Tau, Phf-Tau, Paired Helical Filament-Tau, Neurofibrillary Tangle Protein, Microtubule-Associated Protein Tau, Isoform 4, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.