Biolegend, 434608, LEGEND MAX™ Human IL-27 ELISA Kit, 5precoatedplates

Human IL-27 is a member of the IL-12 cytokine family and is a heterodimeric protein composed of EBI3 (Epstein-Barr virus-induced gene 3) and p28 protein. It is expressed on naïve CD4+ T cells and natural killer cells. IL-27 is produced by antigen-presenting cells to induce proliferation of naive CD4+ T cells and activates human monocytes via STAT1 in addition to suppressing IL-10 production. IL-27 mediates its biological effects through the orphan cytokine receptor WSX-1/TCCR. IL-27 strongly synergizes with IL-12 to support IFN-? production and differentiation of naive CD4+ T cells into T helper type. BioLegend’s LEGEND MAX™ Human IL-27 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-27 specific polyclonal antibody is pre-coated on a 96-well strip-well plate. BioLegend’s LEGEND MAX™ Human IL-27 ELISA Kit is specifically designed for the accurate quantitation of Human IL-27 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Liu L, et al. 2012. J Biol Chem. 287:11899.PubMed He L, et al. 2014. PLoS One. 9:96792. PubMed
Product Citations: Liu L, et al. 2012. J Biol Chem. 287:11899. PubMed Chang K, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.95. PubMed He L, et al. 2014. PLoS One. 9:96792. PubMed
Sensitivity: 11 pg/mL
Standard Range: 0.25-16 ng/mL
Cell Sources: Naïve CD4+ T cells and natural killer cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Gene ID: 246778
UniProt: View information about IL-27 on UniProt.org
Regulatory Status: RUO
Other Names: IL-27 Pre-coated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.