Biolegend, 439008, LEGEND MAX™ Rat IFN-γ ELISA Kit, 5precoatedplates

Interferon-γ (IFN-γ) is the sole member of the type II interferon category. It is a potent multifunctional cytokine involved in both the innate and adaptive (especially cell-mediated) arms of immunity. It is secreted largely by activated lymphocytes, in particular, CD4+ Th1 cells, CD8+ cytotoxic cells, and NK cells. Human IFN-γ is expressed as a pre-cursor protein with a signaling peptide which is cleaved to produce the mature protein of ~17 kD. Its active form is a homodimer, linked by anti-parallel non-covalent interactions between the six α-helices of each monomer. The receptor for IFN-γ is also a homodimer, with α & β subunits (IFNGR1 and IFNGR2, respectively). IFN-γ binds to IFNGR1 very specifically, and the subsequent interaction of IFNGR1 with IFNGR2 activates the Jak/Stat pathway for signal transduction and, ultimately, transcription of effector genes. Rat IFN-γ has 87% amino acid identity with mouse IFN-γ and 39% identity with the human protein. As such, rat IFN-γ can activate mouse cells but not human cells. Originally characterized based on anti-viral properties, IFN-γ also exerts anti-microbial, anti-tumor, immunomodulatory, and pro-inflammatory activities. It is unique among the interferons for its ability to coordinate the transition from innate to adaptive immunity. Among its more significant roles, IFN-γ stimulates macrophage effector functions. It is also the major player in inducing Th1 cell differentiation and provides a positive feedback loop in response to IL-12 stimulation in defining Th1 cells. BioLegend's LEGEND MAX™ Rat IFN-γ ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a rat IFN-γ-specific monoclonal antibody is pre-coated on a 96-well strip-well plate. BioLegend's LEGEND MAX™ Rat IFN-γ ELISA Kit is specifically designed for the accurate quantitation of rat IFN-γ from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
5precoatedplates
Verified Reactivity: Rat
Application: ELISA
Product Citations: Lian YZ, et al. 2022. J Clin Biochem Nutr. 71:229. PubMed Garcia-Cortadella R, et al. 2021. Nat Commun. 0.646527778. PubMed Zhang C, et al. 2022. Front Pharmacol. 12:790072. PubMed
Sensitivity: 3.2 pg/mL
Standard Range: 15.6-1,000 pg/mL
Cell Sources: CD8+ and CD4+ T cells, NK cells
Biology Area: Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 25712
UniProt: View information about IFN-gamma on UniProt.org
Regulatory Status: RUO
Other Names: Rat IFN-γ Pre-coated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.