Biolegend, 443408, LEGEND MAX™ Human NGAL (Lipocalin-2) ELISA Kit, 5precoatedplates

Neutrophil Gelatinase-Associated Lipocalin (NGAL), also known as Lipocalin-2, Siderocalin, 24p3, Uterocalin, or Neu-related lipocalin, is a member of the lipocalin family. It is a secreted protein (25 kD), which captures bacterial siderophores that bind iron with high affinity. NGAL predominantly exists in monomeric form with a small portion in homodimeric and homotrimeric forms. It can also form a heterodimer with MMP-9 and a heterotrimer with MMP-9 and TIMP-1. Human NGAL shares 62% amino acid sequence identity with mouse NGAL. The function of NGAL is diverse and continues to be studied. NGAL acts as a potent anti-bacterial agent via siderophore-mediated iron depletion. NGAL knockout mice exhibit decreased ability to combat bacterial infection. NGAL has been used as an early predictive and prognostic biomarker for acute and chronic kidney injuries. It also has been associated with breast, lung, colon and pancreatic cancer, having diverse, context-dependent effects during tumor development. This may involve cell growth, survival, migration, invasion and angiogenesis. When NGAL form a complex with MMP-9, it protects MMP-9 from degradation, thereby preserving MMP-9’s enzymatic activity, which is involved in various cancers. The LEGEND MAX™ Human NGAL ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human NGAL antibody. The Detection Antibody is a biotinylated mouse monoclonal anti-human NGAL antibody. This kit is specifically designed for the accurate quantitation of human NGAL from cell culture supernatant, serum, plasma, urine, saliva, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Product Citations: Zhang Y, et al. 2022. J Clin Lab Anal. 36:e24526. PubMed Lundquist H, et al. 2022. J Innate Immun. :1. PubMed Ruff WE, et al. 2019. Cell Host Microbe. 26:100. PubMed Li G, et al. 2021. Gut Microbes. 13:1968257. PubMed
Sensitivity: 16.4 pg/mL
Standard Range: 156 - 10,000 pg/mL
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Gene ID: 3934
UniProt: View information about NGAL on UniProt.org
Regulatory Status: RUO
Other Names: Lipocalin2, LCN2, Siderocalin
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.