Biolegend, 444307, LEGEND MAX™ Human Leptin ELISA Kit, 1precoatedplate

Human Leptin is a 167 amino acid protein and approximately 16 kD in mass. Leptin is encoded by the obese (ob) gene and the gene locates on chromosome 7. Leptin is an adipocyte-derived hormone and mainly secreted by adipocyte. Small amounts of Leptin are expressed by the cells in the epithelium of the stomach and in the placenta. Leptin’s effects on body weight are mediated through impacts on hypothalamic center that control feeding behavior, hunger, body temperature, and energy expenditure. Leptin importantly regulates food intake, body weight, metabolism, energy expenditure, reproductive function, and adiposity through the hypothalamic Leptin receptor. Leptin is also the member of long-chain helical cytokine family. The BioLegend LEGEND MAX™ Human Leptin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human Leptin antibody. The detection antibody is a biotinylated mouse monoclonal anti-human Leptin antibody. This kit is specifically designed for the accurate quantitation of human Leptin in serum, plasma, and cell culture supernatant. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Sensitivity: 31.7 ± 12.2 pg/mL
Standard Range: 47 – 3000 pg/mL
Cell Type: Endothelial cells, Fibroblasts
Biology Area: Angiogenesis, Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines, Enzymes and Regulators
Gene ID: NA
UniProt: View information about Leptin on UniProt.org
Regulatory Status: RUO
Other Names: Obese Protein, Obesity Factor, LEPD, OB, OBS
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.