Biolegend, 444309, RAPID MAX™ Human Leptin ELISA Kit, 1precoatedplate

Human Leptin is a 167 amino acid protein and approximately 16 kD in mass. Leptin is encoded by the obese (ob) gene and the gene locates on chromosome 7. Leptin is an adipocyte-derived hormone and mainly secreted by adipocyte; small amounts of Leptin are expressed by the cells in the epithelium of the stomach and in the placenta. Leptin’s effects on body weight are mediated through impacts on hypothalamic center that control feeding behavior, hunger, body temperature, and energy expenditure. Leptin importantly regulates food intake, body weight, metabolism, energy expenditure, reproductive function, and adiposity through hypothalamic Leptin receptor. Leptin is also the member of long-chain helical cytokine family. The BioLegend RAPID MAX™ Human Leptin ELISA Kit is a one step, one wash, 70-minute enzyme-linked immunosorbent sandwich assay (ELISA) with a pre-coated, 96-well strip plate that immobilizes the biotinylated capture antibody. Standards and samples are added into the wells, followed by an antibody cocktail. The antibody cocktail consists of a biotinylated monoclonal capture antibody and an HRP-conjugated monoclonal detection antibody. This kit is specifically designed for the accurate quantitation of human Leptin cell culture supernatant, serum, plasma. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Sensitivity: 19.6 ± 2.4 pg/mL
Standard Range: 62.5 - 4000 pg/mL
Cell Type: Endothelial cells, Fibroblasts
Biology Area: Angiogenesis, Cell Biology, Signal Transduction
Molecular Family: Enzymes and Regulators
Gene ID: NA
UniProt: View information about Leptin on UniProt.org
Regulatory Status: RUO
Other Names: Obese Protein, Obesity Factor, LEPD, OB, OBS