Biolegend, 449307, LEGEND MAX™ Human CD26 (DPP4) ELISA Kit, 1precoatedplate

CD26, also known as Dipeptidyl peptidase-4 (DPP4), is a serine exopeptidase expressed in many different cell types. It can exist as both a membrane protein and an active soluble form. CD26 is involved in many biological processes including but not limited to cell signaling, immune regulation, and apoptosis. For instance, it can act as a co-stimulatory receptor for T cell activation, while being able to cleave multiple chemokines and peptide hormones such as glucagon, shortening their activity. Due to its abundance and multifunctional characteristic, CD26 can be a biomarker for multiple diseases such as immune disfunction, obesity, diabetes, and cancer. The LEGEND MAX™ Human CD26 (DPP4) ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with anti-human CD26 (DPP4) antibody. The detection antibody is a biotinylated anti-human CD26 (DPP4) antibody. This kit is specifically designed for the accurate quantitation of human CD26 (DPP4) in serum, plasma, saliva, urine, and cell culture supernatant. It is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Sensitivity: 21.3 + 4.5 pg/mL
Standard Range: 125 - 8000 pg/mL
Cell Type: Antigen-presenting cells, Epithelial cells, T cells
Biology Area: Cancer Biomarkers, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family: CD Molecules
Gene ID: P27487
UniProt: View information about CD26 on UniProt.org
Regulatory Status: RUO
Other Names: Depeptidyl Peptidase 4, ADCP2, Adenosine Deaminase Complexing Protein 2
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.