Biolegend, 480053, MojoSort™ Human NK Cell Isolation Kit, 20tests

Non-natural killer (NK) cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched NK cells (CD56 + , CD3 - ) are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
20tests
Verified Reactivity: Human
Formulation: Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation: The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.Strepavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling: Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application: Cell Separation (MojoSort™) - Quality tested
Recommended Usage: 10 µl of antibody cocktail for 1 x 10^7 cells in 100 µl of buffer. 10 µl of Streptavidin Nanobeads for 1 x 10^7 cells in 100 µl of buffer.
Application Notes: This kit is designed for the isolation of untouched Natural Killer (NK) Cells from peripheral blood mononuclear cells (PBMCs). Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Product Citations: Jo DH, et al. 2023. Mol Ther Methods Clin Dev. 29:173. PubMed Hobson SR, et al. 2018. J Pineal Res. e12508:65. PubMed Zheng B, et al. 2021. Int J Mol Sci. 22: . PubMed Harmon C, et al. 2019. Front Immunol. 10:1502. PubMed Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed Zhao S, et al. 2021. Arthritis Rheumatol. Online ahead of print.. PubMed Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Gene ID: NA
UniProt: View information about NK on UniProt.org
Regulatory Status: RUO
Q: Is there a way to detach your magnetic particles from the cell surface?
A: MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
Q: What is the size of your magnetic particles?
A: MojoSort Nanobeads: the average diameter is approximately 130 nm.

MojoSort Microbeads: the average diameter is approximately 1 μm.
Q: Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
A: MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
Q: What antibodies are present in the depletion cocktails provided for isolation kits?
A: Please contact our technical service team for further assistance.