Biolegend, 501701, Purified anti-human IL-12/IL-23 p40 (monomer, homodimer) Antibody, 50μg

The C8.3 antibody reacts with human interleukin-12 and interleukin-23 (IL-12/IL-23) p40 monomer or homodimer. This antibody does not react with either the p35 subunit of IL-12 or the p19 subunit of IL-23, or the p40 protein when it is heterodimerized with p35 (the IL-12 p70) or p19 (IL-23 p60). The C8.3 antibody cannot neutralize the antagonistic activity of IL-12 p40.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: COS-expressed p40 subunit of human IL-12
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of IL-12 (p40) recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate.
Application Notes: ELISA or ELISPOT Capture: The purified C8.3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C8.6 antibody (Cat. No. 508802/508805) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. This assay detects specifically human IL-12/IL-23 p40 protein, without interference by IL-12 p70 or IL-23 p19 proteins. Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): D'Andrea A, et al. 1992. J. Exp. Med. 176:1387. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041.
Product Citations: Stensland ZC, et al. 2022. iScience. 25:103626. PubMed Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed
RRID: AB_2255186 (BioLegend Cat. No. 501702)
Structure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by hu
Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets: T cells, NK cells, PBL, mature dendritic cells
Receptors: IL-12Rβ1 binds p40 from both IL-12 and IL-23; dimeric with IL-12Rβ2 binds p35; IL-23 specific receptor subunit, IL-23R binds p19
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992. Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993. Immunol. Today. 14:335. 5. Oppmann B, et al. 2000. Immunity. 13:715. 6. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002. J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002. J. Immunol. 168:5448. 9. Lankford CS, et al. 2003. J. Leukoc. Biol. 73:49.
Regulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function
Gene ID: 3593
UniProt: View information about IL-12/IL-23 p40 on UniProt.org
Clone: C8.3
Regulatory Status: RUO
Other Names: Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype: Mouse IgG1, κ