Biolegend, 503304, Biotin anti-human NGAL (Lipocalin-2) Antibody, 500μg

NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils. NGAL is a critical component of innate immunity because of its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising markers for renal epithelial injury and cardiovascular disease (CVD).
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human NGAL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.15 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 2500 to 39.1 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
Application Notes: ELISA Detection: The biotinylated M049D6 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M0415B7 (Cat. No. 661902) antibody as the capture antibody. Note: For testing human NGAL in cell culture supernatant, serum, plasma, urine, saliva, and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443407 & 443408) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209.Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.Flo TH, et al. 2004. Nature 432:917Goetz DH, et al. 2002. Mol. Cell. 10:1033.Leung L, et al. 2012. PLoS One 7:e46677Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.
RRID: AB_2650710 (BioLegend Cat. No. 503304)
Structure: 193 amino acid monomer with a predicted molecular weight of approximately 22 kD.
Distribution: Secreted by epithelial cells, macrophages, neutrophils. Increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.
Function: Apoptosis, innate immunity, renal development.
Interaction: Disulfide-linked with MMP9
Ligand/Receptor: SLC22A17 (24p3R)
Cell Type: Epithelial cells, Macrophages, Neutrophils
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Gene ID: 3934
UniProt: View information about NGAL on UniProt.org
Clone: M049D6
Regulatory Status: RUO
Other Names: Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.