Biolegend, 503510, GoInVivo™ Purified anti-mouse/rat IL-1β Antibody, 100mg

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The B122 antibody reacts with the precursor and mature secreted forms of mouse IL-1β. This antibody also recognizes rat IL-1β. The B122 antibody can neutralize the bioactivity of natural or recombinant IL-1β.
100mg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: E. coli -expressed, recombinant mouse IL-1β
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation: The GoInVivo™ antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application: ELISA Capture - Quality tested ELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA or ELISPOT Capture3: The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Certain formats of clone B122 have been reported for in vitro neutralization of IL-1ß bioactivity1, in vivo cytokine depletion5, immunohistochemical staining of frozen sections6, Western blotting7, and the study of endotoxin shock8.
Additional Product Notes: GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.
Application References(PubMed link indicates BioLegend citation): Hogquist K, et al. 1991. J. Immunol. 146:1534. Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut) Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture) Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011. Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion) Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC) Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB) Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed
RRID: AB_2888752 (BioLegend Cat. No. 503507) AB_2888752 (BioLegend Cat. No. 503508) AB_2888752 (BioLegend Cat. No. 503509) AB_2888752 (BioLegend Cat. No. 503510) AB_2888752 (BioLegend Cat. No. 503511) AB_2888752 (BioLegend Cat. No. 503512)
Structure: Cytokine; 17.5 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets: B cells, T cells, monocytes
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID: 16176
UniProt: View information about IL-1beta on UniProt.org
Clone: B122
Regulatory Status: RUO
Other Names: Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype: Armenian Hamster IgG