Biolegend, 507211, Alexa Fluor® 488 anti-human Granzyme A Antibody, 25tests

Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human Granzyme A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.
Application References(PubMed link indicates BioLegend citation): Trimble L, et al. 1998. Blood 91:585. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285. Raqib R, et al. 2002. Infect. Immun. 70:3199. Chen H, et al. 2005. J. Immunol. 175:591.
Product Citations: Zhang Q, et al. 2022. Nucleic Acids Res. Online ahead of print. PubMed Buggert M, et al. 2014. PLoS Pathog. 10:1004251. PubMed Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
RRID: AB_528909 (BioLegend Cat. No. 507212)
Structure: Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
Ligand/Receptor: Assisted by perforin
Bioactivity: Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Cell Sources: Cytotoxic T cells, NK cells
Cell Targets: Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Receptors: Assisted by perforin
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: 1. Brune J, et al. 1986. Nature 322:268. 2. Fan Z, et al. 2003. Nature Immunol. 4:145. 3. Fan Z, et al. 2003. Cell 112:659. 4. Masson D, et al. 1987. Cell 49:679.
Gene ID: 3001
UniProt: View information about Granzyme A on UniProt.org
Clone: CB9
Regulatory Status: RUO
Other Names: Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
Isotype: Mouse IgG1, κ