Biolegend, 509501, Biotin anti-human IL-1 RA Antibody, 50μg

Interleukin-1 receptor antagonist (IL-1RA) is composed of three isoforms, one secreted (sIL-1Rα/IL-1Rα1), two intracellular (IL-1Rα2/IL-1Rα3) and produced by monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma cells, keratinocytes, and chondrocytes. It is upregulated by IL-1β, IL-4, IL-6 and downregulated by glucocorticoids. The Poly5095 polyclonal antibody reacts with human interleukin-1 receptor antagonist (IL-1RA).
50μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Recombinant human IL-1 receptor antagonist
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection: The biotinylated Poly5095 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified JK1RA-1 (Cat. No. 509902) antibody as the capture antibody.This product may contain other non-IgG subtypes.
RRID: AB_315540 (BioLegend Cat. No. 509502)
Structure: Cytokine; three isoforms, one secreted (sIL-1Rα/IL-1Rα1), two intracellular (IL-1Rα2/IL-1Rα3); 17 kD (Mammalian)
Bioactivity: Storage forms that are released upon cell death to limit inflammation caused by cell debris; competitive inhibitor of IL-1 receptor binding; antagonizes IL-1 activities without having agonist effects; does not initiate intracellular signal transduction pr
Cell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma cells, keratino
Cell Targets: B cells, T cells, monocytes
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Carter D, et al. 1990. Nature 344:633. 2. Chirivi R, et al. 1993. Cancer Res. 53:5051. 3. Holtkamp G, et al. 1999. Eur. J. Immunol. 29:215. 4. Kim J, et al. 2003. Gene Ther. 10:1543.
Regulation: Upregulated by IL-1β, IL-4, IL-6; downregulated by glucocorticoids
Gene ID: 3557
UniProt: View information about IL-1RA on UniProt.org
Clone: Poly5095
Regulatory Status: RUO
Other Names: Interleukin-1 receptor antagonist, IL-1 inhibitor, IL-1 receptor antagonist protein (IRAP), IL-1 inhibitory activity, ILS, MG50, IL-1RN
Isotype: Rabbit Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.