Biolegend, 509802, Biotin anti-mouse/human NGF Antibody, 500μg

Nerve growth factor (NGF) is a small secreted protein that is important for the growth, maintenance, and survival of certain target neurons (nerve cells). NGF consists of three types of subunits, α, β and γ, which specifically interact to form a 7S, 130,000 molecular weight complex. This complex contains two identical 118-amino acid β-chains, which are solely responsible for nerve growth stimulating activity of NGF. NGF is essential for the development and phenotypic maintenance of neurons in the peripheral nervous system (PNS) and for the functional integrity of cholinergic neurons in the central nervous system (CNS).
500μg
Verified Reactivity: Human
Reported Reactivity: Mouse, Cow, Viper
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: recombinant human NGF (c-terminus)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1 - 3 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Additionally, BioLegend recommends the usage of Tris-Buffered Saline (TBS), pH = 7.2 for all washes and incubations, except substrate buffer. Do not use PBS as results may vary dramatically.
Application Notes: ELISA Detection (mouse): The biotinylated JKhNGF-2 antibody is useful as the detecting antibody in a sandwich ELISA assay, when used in conjunction with the purified JKmNGF-1 antibody (Cat. No. 509702) as the capture antibody for detecting mouse NGF. ELISA Detection (human): The biotinylated JKhNGF-2 antibody is useful as the detecting antibody in a sandwich ELISA assay, when used in conjunction with the purified JKhNGF-1 antibody (Cat. No. 509602) as the capture antibody for detecting human NGF.
RRID: AB_2152401 (BioLegend Cat. No. 509801) AB_2152400 (BioLegend Cat. No. 509802)
Structure: Kallikrein family of serine proteases; dimer; 13.5 kD (Mammalian)
Bioactivity: Enhances survival, growth, neurotransmitter biosynthesis of sympathetic, sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation, survival B lymphocytes; possible role in allergy, tissue repair
Cell Sources: Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells, eosinophils
Cell Targets: Neurons, B cells, mast cells, eosinophils
Receptors: Low-affinity NGF receptor (LNGFR, p75 LNGFR, fast NGF receptor); high-affinity NGF receptor (trkA)
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Levi A, et al. 1991. Annu. Rev. Pharmacol. Toxicol. 31:205. 3. Varon S, et al. 1991. Adv. Exp. Med. Biol. 296:267. 4. Micera A, et al. 2003. Cytokine Growth Factor Rev. 14:369.
Regulation: Upregulated by glutamate, vitamin D3, IL-6, FGF-basic, astrocyte-specific IL-1, TNF-α, PDGF, TGF-β; downregulated by GABAergic neuronal activity, glucocorticoids, Schwann cell-specific TGF-β
Gene ID: 180494803
UniProt: View information about NGF on UniProt.org
Clone: JKhNGF-2
Regulatory Status: RUO
Other Names: Nerve growth factor, NGF, β-NGF
Isotype: Mouse IgG1
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.