Biolegend, 511501, Purified anti-human IL-8 Antibody, 50μg

IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The H8A5 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA capture antibody.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection: The purified H8A5 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated E8N1 (Cat. No. 511404) antibody as the detection antibody. Note: For testing human IL-8 in serum or plasma, BioLegend's ELISA Max™ Sets are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Sauer U, et al. 2011. Anal Biochem. 419:46. PubMed Domnanich P, et al. 2011. Biosens Bioeletron. 26:2259. PubMed Sauer U, et al. 2012. J Immunol Methods. 378:44. PubMed
Product Citations: Gogalic S, et al. 2018. Biosensors (Basel). 9:1593. PubMed Jarrar W, et al. 2015. PLoS One. e0124408:10. PubMed Marroquin Belaunzaran O, et al. 2015. PLoS One. e0130811:10. PubMed Naikawadi RP, et al. 2016. JCI Insight. e86704:1. PubMed Sauer U, et al. 2011. Anal Biochem. 419:46. PubMed Domnanich P, et al. 2011. Biochem Biophys Res Commun. 26:2559. PubMed Sauer U, et al. 2012. J Immunol Methods. 378:44. PubMed
RRID: AB_893455 (BioLegend Cat. No. 511501) AB_893456 (BioLegend Cat. No. 511502)
Structure: CXC chemokine; 8.4 kD (Mammalian)
Ligand/Receptor: CXCR1 (CDw128); CXCR2
Bioactivity: Neutrophil/basophil chemoattractant; activates neutrophils, angiogenic factor
Cell Sources: Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Cell Targets: Neutrophils, basophils, lymphocytes
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97. 3. Schröder J, et al. 1992. Immunology Ser. 57:387. 4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.
Regulation: Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-ß, glucocorticoids, vitamin D3
Gene ID: 3576
UniProt: View information about IL-8 on UniProt.org
Clone: H8A5
Regulatory Status: RUO
Other Names: Interleukin-8, Neutrophil activating protein-1, Monocyte-derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Leukocyte adhesion inhibitor (LAI), Granulocyte chemotactic protein (GCP), CXCL8
Isotype: Mouse IgG2a, κ