Biolegend, 511704, Biotin anti-human IL-1β Antibody, 500μg

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection, ELISPOT Detection - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. Clone H1b-98 is useful as the detection antibody in a sandwich ELISA when used in conjunction with the purified H1b-27 antibody (Cat. No. 511601 or 511604). For ELISA detection applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 31.3 pg/mL are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2.0 - 8.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Haug V, et al. 2020. Transplantation. e260:104. PubMed Croden J, et al. 2021. PLoS One. 16:e0258208. PubMed Bando H, et al. 2019. Front Cell Infect Microbiol. 9:140. PubMed Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed Watkins H, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.010. PubMed
RRID: AB_1236448 (BioLegend Cat. No. 511703) AB_1236449 (BioLegend Cat. No. 511704)
Structure: Cytokine; 17.3 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Cell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cell Targets: B cells, T cells, monocytes
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID: 3553
UniProt: View information about IL-1beta on UniProt.org
Clone: H1b-98
Regulatory Status: RUO
Other Names: Interleukin-1β, H1, OAF, Catabolin, IFNb-inducing factor(il-1b,il1b)
Isotype: Mouse IgG2b, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Q: Does this clone recognize the pro or mature form of IL-1 beta?
A: The monoclonal anti-human IL-1b antibody clone H1b-98 was generated and screened using the mature form of IL-1b as the immunogen. Since the pro-form contains the mature sequence, it is believed that the antibody also recognize pro-IL-1beta. Similarly, we believe clone JK1B-1 should also recognize both pro and mature forms of IL-1b. However, this information has not been confirmed with in-house testing data.