Biolegend, 511801, Purified anti-mouse IL-12 (p70) Antibody, 50μg

Interleukin 12 (IL-12) is a heterodimeric pleiotropic 70 kD (p70) cytokine consisting of a 40 kD (p40) subunit and a 35 kD (p35) subunit. IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells, and macrophages in response to pathogens or upon interaction with activated T cells.IL-12 p70 stimulates cytotoxic T, NK and LAK cell proliferation, andinduces IFN-γ and TNF production in resting and activated T and NK cells.It has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO expressed, recombinant mouse IL-12 p70
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12(p70) recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture: The purified C18.2 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated C17.8 antibody as detection antibody and recombinant mouse IL-12 p70 as the standard.
Product Citations: Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed Liang D, et al. 2018. Front Immunol. 8:1801. PubMed Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed Jin X, et al. 2020. Cell Reports. 31(8):107690. PubMed Zhou Y, et al. 2018. Cell Mol Immunol. 15:246. PubMed Liu C, et al. 2019. Theranostics. 9:1369. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
RRID: AB_2123771 (BioLegend Cat. No. 511801) AB_2123769 (BioLegend Cat. No. 511802)
Structure: heterodimeric cytokine composed of p40 subunit shared with IL-23 and a specific p35 subunit
Distribution: Secreted by dendritic cells, monocytes/macrophages, B cells, T cells
Function: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Interaction: T cells, NK cells
Ligand/Receptor: IL-12Rβ1 binds p40 shared with IL-23; dimeric with IL-12Rβ2 binds p35
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes.
Cell Type: Dendritic cells, Monocytes, Macrophages, B cells, T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lankford CS, et al. 2003 J. Leukoc. Biol.. 73:49.2. Parham C, et al. 2002 J. Immunol.. 168:5699.3. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.5. Quesniaux V. 1992. Research Immunol. 143:385.6. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
Gene ID: 1615916160
UniProt: View information about IL-12 p70 on UniProt.org
Clone: C18.2
Regulatory Status: RUO
Other Names: Interleukin-12 p70, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF)
Isotype: Rat IgG2a, κ