Biolegend, 512201, Purified anti-human TSLP Antibody, 50μg

Thymic Stromal Lymphopoietin (TSLP) is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naïve T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFα) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation. Recent studies revealed that expression of TSLP has a direct link to the pathogenesis of allergic inflammation such as atopic dermatitis and asthma via activating dendritic cells and mast cells which trigger inflammatory Th2 response characterized by high TNF-alpha and little IL-10 production.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human TSLP
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of TSLP recombinant protein ranging from 200 to 3.1 ng/ml are recommended for each ELISA plate.
Application Notes: ELISA Capture: Purified and Ultra-LEAF™ purified 15B11.3 antibody are useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5159 antibody (Cat. No. 515903) as the detecting antibody.
Application References(PubMed link indicates BioLegend citation): Yeh CY, et al. 2013. PLoS One. 8:e55472. (Neut) PubMed
RRID: AB_940352 (BioLegend Cat. No. 512202)
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Liu YJ. 2007. J. Allergy Clin. Immunol. 120:238. 2. Nagata Y. 2007. Int. Arch. Allergy Immunol. 144:305. 3. Jiang Q, et al. 2007 BMC Immunol. 8:11. 4. Kato A. et al. 2007. J. Immunol. 179:1080. 5. Rochman I, et al. 2007 J. Immunol. 178:6720. 6. Reche PA, et al. 2001. J. Immunol. 167:336. 7. Pandey A, et al. 2000. Nat. Immunol. 1:59. 8. Park LS, et al. 2000. J Exp. Med. 192:659.
Gene ID: 85480
UniProt: View information about TSLP on UniProt.org
Clone: 15B11.3
Regulatory Status: RUO
Other Names: Thymic stromal lymphopoietin (TSLP)
Isotype: Mouse IgG1, κ