Biolegend, 512321, Brilliant Violet 421™ anti-human IL-17A Antibody, 25tests

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4 + Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4 + helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human IL-17A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Product Citations: Cao J, et al. 2023. BMC Immunol. 24:6. PubMed Imai H, et al. 2023. iScience. 26:106822. PubMed Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed Asashima H, et al. 2022. J Clin Invest. 132: . PubMed Barros-Martins J, et al. 2021. Nat Med. 27:1525. PubMed Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed Booth JS, et al. 2019. Hum Vaccin Immunother. :1. PubMed Nicholas DA et al. 2019. Cell Metab. 30(3):447-461 . PubMed Bailur JK, et al. 2020. JCI Insight. 5:00. PubMed Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed Wood MP, et al. 2020. J Infect Dis. 222:44. PubMed Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed Shen X, et al. 2021. Front Immunol. 12:710750. PubMed Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed Khaitan A, et al. 2016. PLoS One. 11: 0161786. PubMed Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
RRID: AB_10899566 (BioLegend Cat. No. 512321) AB_11218604 (BioLegend Cat. No. 512322)
Structure: Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources: Activated T cell subpopulation
Cell Targets: Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors: IL-17R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Hirota K, et al. 2007. J. Exp. Med. 204:41. 2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169. 3. Witowski J, et al. 2007. Kidney Int. 71:514. 4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255. 5. Hymowitz S, et al. 2001. EMBO J. 20:5332.
Gene ID: 3605
UniProt: View information about IL-17A on UniProt.org
Clone: BL168
Regulatory Status: RUO
Other Names: Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.