Biolegend, 513904, LEAF™ Purified anti-rat IL-10 Antibody, 100μg

IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. It inhibits interferon IFNγ synthesis in Th1 cell, and therefore it was initially called cytokine synthesis inhibiting factor (CSIF). Macrophages are the main source of IL-10 and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-κB dependent), tumor necrosis factor TNFα (via TNF receptor p55, NF-kB-dependent), catecholamines, and IL-1. IL-10 controls inflammatory processes by suppressing the expression of proinflammatory cytokines, chemokines, adhesion molecules, as well as antigen-presenting and costimulatory molecules in monocytes/macrophages, neutrophils, and T cells. IL-10 inhibits the production of proinflammatory mediators by monocytes and macrophages such as endotoxin- and IFNγ-induced release of IL-1α, IL-6, IL-8, G-CSF, GM-CSF, and TNFα. In addition, it enhances the production of anti-inflammatory mediators such as IL-1RA and soluble TNFα receptors. IL-10 inhibits the capacity of monocytes and macrophages to present antigen to T cells. This is realized by down-regulation of constitutive and IFNγ-induced cell surface levels of MHC class II, of costimulatory molecules such as CD86 and of some adhesion molecules such as CD58.
100μg
Verified Reactivity: Rat
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant Rat IL-10
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.1 EU/μg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by the neutralization of mouse IL-6 production induced by recombinant rat IL-10 at 20 ng/mL on Raw264.7 cells. ND50 range: 0.1 - 1.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3716904 (BioLegend Cat. No. 513904)
Structure: Monomer
Distribution: IL-10 is produce by Th2 cells, macrophages, DCs, B cells, CD8+ T cells, regulatory T cells (Tregs), Th1 cells and Th17 cells. In addition, IL-10 is expressed by monocytes, B cells, eosinophils, and mast cells.
Function: IL-10 is an immunoregulatory cytokine. Its main function is the limitation and termination of inflammatory responses and the regulation of differentiation and proliferation of several immune cells such as T cells, B cells, natural killer cells, antigen-presenting cells, mast cells, and granulocytes.
Interaction: IL-10R is expressed in monocytes, NK, B, and T cells. In addition, Langerhans cells, dermal dendritic cells, eosinophils, mast cells, and endothelial cells can respond to IL-10.
Ligand/Receptor: IL-10 binds to their receptors IL-10R1 and IL-10R2, and initiates a STAT3-dependent signaling cascade.
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: Feng L, et al. 1993. Bichem Biophys Res Commun. 192:452-8. Fiorentino DF, et al. 1989, J Exp Med. 170:2081-95. Ho AS, et al. 1993. P Natl Acad Sci USA. 90:11267. Hart PH, et al. 1996. J Immunol. 157:3672-80. Asadullah K, et al. 2003. Pharmacol Rev. 55:241-69. Mosser DM and Zhang X. 2008. Immunol Rev. 226:205. Maynard CL and Weaver CT. 2008. Immunol. Rev. 226:219.
Gene ID: 25325
UniProt: View information about IL-10 on UniProt.org
Clone: Poly23A23
Regulatory Status: RUO
Other Names: CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL-10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
Isotype: Goat Polyclonal IgG
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.