Biolegend, 514302, Purified anti-human/mouse IL-21 Antibody, 500μg

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses.  These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells.  It can also be proapoptotic for B cells and NK cells.  Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces T(H)17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
500μg
Verified Reactivity: Human
Reported Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human IL-21
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store frozen between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product weakly reacts with Mouse.
Product Citations: Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
RRID: AB_1227666 (BioLegend Cat. No. 514302)
Distribution: Th17
Function: Immunomodulation
Cell Sources: NK-T cells and T cells
Cell Targets: cytotoxic T cells (CTL) and Natural Killer (NK) cells, that can destroy malignant or infected cells
Receptors: IL-21R
Cell Type: Th17
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Nurieva R,et al. 2007. Nature 448:416. 2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856. 3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144. 4. Asao H, et al. 2001. J. Immunol. 167:1. 5. Parrish-Novak J, et al. 2000. Nature 408:57.
Gene ID: 5906760505
UniProt: View information about IL-21 on UniProt.org
Clone: 7H20-I19-M3
Regulatory Status: RUO
Other Names: Interleukin 21
Isotype: Mouse IgG1, κ