Biolegend, 514801, Purified anti-human FGF-basic Antibody, 50μg

Fibroblast growth factor-basic (also known as FGF-b, FGF-2) is a heparin-binding growth factor, which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo . FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The BHFB15 antibody reacts with human fibroblast growth factor - basic (FGF-basic).
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human FGF-basic.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 2 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture: The purified BHFB15 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JKFb-2 antibody as the detecting antibody.
RRID: AB_2561316 (BioLegend Cat. No. 514801) AB_2561317 (BioLegend Cat. No. 514802)
Structure: 17.2 kD (Mammalian)
Distribution: Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartiliage, endothelial cells, and epithelial cells.
Function: Released after tissue injuries and during the inflammatory process.
Interaction: Fibroblasts, myobalasts, osteoblasts, neuronal cells, endothelial cells, kerintocytes, chrondrocytes, astrocytes, oligodendrocytes, and the smooth muscle.
Ligand/Receptor: FGFR1-1 (flg); FGFR-2 (bek, K-sam); FGFR-4 (flig-2); low affinity coreceptor heparin sulfate, and heparin sulfate proteoglycans required for full activity.
Cell Type: Monocytes, Epithelial cells, Endothelial cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald KA, et al. 2013. The Cytokine Factsbook. Acadmic Press. San Diego. 2. Klagsbrun M, 1992. Semin. Cancer Biol. 3:81. 3. Goldfarb M. 1990. Cell Growth Factor. 1:439. 4. Sensenbrenner M. 1993. Prog. Neurobiol. 6:683.
Gene ID: 2247
UniProt: View information about FGF-basic on UniProt.org
Clone: BHFB15
Regulatory Status: RUO
Other Names: Fibroblast Growth Factor-basic, bFGF, FGF-2, FGF2, Heparin-binding growth factor
Isotype: Mouse IgG1, κ