Biolegend, 516303, Biotin anti-human IL-33 Antibody, 50μg

IL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1α or IL-1β stimulation. IL-33 interacts with IL1RL1/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.
50μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Human IL-33, amino acids Ser112-Thr270 (Accession# NM_ 033439) was expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 1% BSA (USA origin) and 0.09% sodium azide.
Preparation: The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.125-0.5 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000 to 15.6 pg/ml are recommended.
Application Notes: ELISA Detection: The biotinylated Poly5163 antibody is useful as a detection antibody in a sandwich ELISA assay, when used in conjunction with purified Poly5163 (Cat. No. 516301) antibody as the capture antibody and recombinant human IL-33 (Cat. No. 581809) as the standard.
RRID: AB_2561413 (BioLegend Cat. No. 516303)
Structure: 270 aa with predicted molecular weight of 30 kD.
Distribution: Full length IL-33 localizes to the nucleus in HUVECs and transfectants C-terminal fragment, corresponding mature IL-33 is secreted.
Function: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines.
Interaction: Binding to IL1RL1/ST2 and IL-1 receptor accessory protein.
Cross: In sandwich ELISA, no cross-reactivity with mouse or rat IL-33.
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lohning M, et al. 1998. P. Natl. Acad. Sci. USA 95:693. 2. Onda H, et al. 1999. J. Cerebr. Blood F. Met. 19:1279. 3. Schmitz J, et al. 2005. Immunity 23:479. 4. McLaren JE, et al. 2010. J. Immunol. 185:1222.
Gene ID: 90865
UniProt: View information about IL-33 on UniProt.org
Clone: Poly5163
Regulatory Status: RUO
Other Names: Interleukin-33
Isotype: Goat Polyclonal Ig
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.