Biolegend, 516501, Purified anti-mouse IL-33 Antibody, 100μg

IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Mouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by antigen affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000-15.6 pg/ml are recommended.
Application Notes: ELISA Capture: The purified Poly5165 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5165 antibody (Cat. No. 516503) as the detecting antibody and recombinant mouse IL-33 (Cat. No. 580509) as the standard.
Application References(PubMed link indicates BioLegend citation): Gao X, et al. 2015. J Immunol. 194:438. PubMed
Product Citations: Gao X, et al. 2015. J Immunol. 194:438. PubMed
RRID: AB_2561333 (BioLegend Cat. No. 516501)
Distribution: High levels of mouse IL-33 mRNA are detected in the stomach, lung, spinal cord, brain, and skin. Expression within these organs is restricted to a few cell types, such as epithelial cells, fibroblasts, and smooth muscle cells. In addition, mouse IL-33 mRNA is found in resting dendritic cells and activated macrophages. Endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues.
Function: IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-a and IL-1b are activators of IL-33 transcription in fibroblasts and keratinocytes.
Ligand/Receptor: IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein).
Cell Type: Epithelial cells, Fibroblasts
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Schmitz J, et al. Immunity 2005 23:479 2. Barksby HE, et al. C Exp Immunol 2007 149:217 3. Arend WP, et al. ImmRev 2008 223:20 4. Suzukawa M, et al. J. Immunol. 2008 181:5981 5. Moussio C, et al. PlosOne 2008 10:e3331.
Gene ID: 77125
UniProt: View information about IL-33 on UniProt.org
Clone: Poly5165
Regulatory Status: RUO
Other Names: Il-1f11, Nuclear factor from high endothelial venules (NF-HEV)
Isotype: Goat Polyclonal Ig