Biolegend, 516604, Alexa Fluor® 488 anti-human IL-17F Antibody, 100tests

IL-17F is part of the IL-17 cytokine family which consists of at least seven structurally related proteins (IL-17A, B, C, D, E, F, and A/F). IL-17F homodimer adopts a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for inter-chain bonding. IL-17F is most closely related to IL-17A, sharing 50% amino acid sequence homology. It is expressed by Th17 cells, along with IL-17A homodimer and IL-17A/F heterodimer. IL-17F has been shown to inhibit endothelial cell angiogenesis and induce increased production of IL-2, TGF-β, and MCP-1, playing a critical role in the regulation of inflammatory reactions and in a variety of auto-immune diseases. The IL-17A/F heterodimer consists of the monomeric units of IL-17A and IL-17F. It is a naturally expressed and biologically active molecule. While much has been studied about IL-17A and IL-17F, it is still unclear how each distinctively induces the inflammatory response, and what role IL-17A/F also plays in it. It is known that all three cytokines are produced in different amounts, and each has different degrees of pro-inflammatory effects. All seem to interact with the IL-17RA/IL-17RC receptor complex, and signal via the NF-κB pathway to induce secretion of inflammatory cytokines and recruit granulocytes. BioLegend's purified Poly5166 antibody is IL-17F specific and can be paired with biotinylated Poly5166 to recognize IL-17F or with biotinylated anti-human IL-17A clone BL23 to recognize the IL-17A/F heterodimer.
100tests
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: The pure Poly5166 (Cat. No. 516601) antibody is useful as the capture antibody in a sandwich ELISA assay. Poly5166 can be used as a poly-poly pair with biotinylated Poly5166 to recognize human IL-17F, or it can be used as a poly-mono pair with biotinylated BL23 (Cat. No. 512705) to recognize the human IL-17A/F heterodimer (Cat. No. 580609).
Product Citations: Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed Nguyen DX, et al. 2021. iScience. 24(9):102973. PubMed Hernndez DC, et al. 2021. Immunity. :. PubMed Minns D, et al. 2021. Nat Commun. 12:1285. PubMed Jiang J, et al. 2016. Sci Rep. 6: 32320. PubMed
RRID: AB_10730721 (BioLegend Cat. No. 516603) AB_10720816 (BioLegend Cat. No. 516604)
Structure: Homodimer
Distribution: T cells, Th17 cells, memory CD4 T cells, gamma delta T cells, and epithelial cells, activated human mast cells, and basophils
Function: IL-17F induces various cytokines, chemokines, and adhesion molecules, including IL-6, IL-8, CXCL1, CXCL5, transforming growth factor-b (TGF-b), CCL2, G-CSF, granulocyte macrophage-colony forming factor (GM-CSF) and intracellular adhesion molecule-1 (ICAM-1), in airway epithelial cells, vein endothelial cells, and fibroblasts.
Interaction: IL-17F might act primarily on parenchymal cells such as fibroblasts, epithelial cells, and endothelial cells.
Ligand/Receptor: IL-17F utilizes IL-17RA and IL-17RC
Cell Type: Basophils, Epithelial cells, Mast cells, T cells, Th17
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: Starnes T, et al. 2001. J. Immunol. 167:4137. Kawaguchi M, et al. 2001. J. Immunol. 167:4430. Hizawa N, et al. 2006. Clin Experimental Allergy 36:1109. Seiderer J, et al. 2008. Inflamm Bowel Dis. 14:437. Wu X, et al. 2010 Int J Cancer 127:86.
Gene ID: 112744
UniProt: View information about IL-17F on UniProt.org
Clone: Poly5166
Regulatory Status: RUO
Other Names: Interleukin-17F, IL-17F
Isotype: Goat Polyclonal Ig