Biolegend, 516704, PE anti-human IL-21 Antibody, 100tests

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine primarily produced by NKT cells and CD4 + T cells, particularly the inflammatory Th17 subset. It exerts pleiotropic effects on both innate and adaptive immune responses. These include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. IL-21 also has proapoptotic effects on B cells and NK cells. Recent studies have shown that IL-21 functions as an autocrine cytokine, strongly inducing Th17 differentiation and suppressing Foxp3 expression, making it a potential therapeutic target for treating cancer and inflammatory diseases.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Rohman M, et al. 2007. FEBS Lett. 581:4001. Onoda T, et al. 2007. Int. Immunol. 19:1191.
Product Citations: Pissani F, et al. 2018. J Virol. 92:e01143. PubMed
RRID: AB_2125257 (BioLegend Cat. No. 516703) AB_2125256 (BioLegend Cat. No. 516704)
Distribution: Th17
Function: Immunomodulation
Cell Sources: NK-T cells and T cells
Cell Targets: cytotoxic T cells (CTL) and Natural Killer (NK) cells, that can destroy malignant or infected cells
Receptors: IL-21R
Cell Type: Th17
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Nurieva R. 2007. Nature 448:416. 2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856. 3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144. 4. Asao H, et al. 2001. J. Immunol. 167:1. 5. Parrish-Novak J, et al. 2000. Nature 408:57.
Gene ID: 59067
UniProt: View information about IL-21 on UniProt.org
Clone: 4BG1
Regulatory Status: RUO
Other Names: Interleukin 21
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.