Biolegend, 517502, Purified anti-mouse IL-22 Antibody, 100μg

IL-22, a cytokine structurally related to IL-10, was originally identified in the mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as IL-TIF (IL10-related T cell-derived inducible factor). Mouse IL-TIF consists of 179 amino acids, including four cysteins, and has a predicted molecular weight of 20 kD.  Biological activity of IL-22 is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor families. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on non-immune cells to regulate local tissue inflammation. As a product of the recently identified T helper 17 lineage of CD4(+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in dysregulated inflammation observed in autoimmune diseases.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mature recombinant protein mouse Interleukin-22 (Leu34-Val179)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg/ml dilution buffer for each mini-gel.
Application Notes: Clone BL35175 has been shown to be useful for detecting mouse and human IL-22 by Western blotting.
Product Citations: Ogita T, et al. 2021. Front Nutr. 8:701466. PubMed Ke Y, et al. 2011. J Immunol. 187:2130. PubMed Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
RRID: AB_10695475 (BioLegend Cat. No. 517501) AB_10694243 (BioLegend Cat. No. 517502)
Structure: 179 aa with with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein.
Distribution: Secreted, Th17
Function: Cytokine that contributes to the inflammatory response in vivo.
Ligand/Receptor: Binds to IL-22 receptor complex that consists of two receptor subunits, IL22R and IL10Rβ.
Cell Type: Th17
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Dumoutier L, et al. 2000. J. Immunol. 164:1814. 2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335. 3. Jones BC, et al. 2008. Structure 16:1333. 4. Sonnenberg GF, et al. 2010. J. Exp. Med. 207:1293. 5. Kotenko SV, et al. 2001. J. Immunol. 166:7096. 6. Chang C, et al. 2006. Cell Research 16:902.
Gene ID: 50929
UniProt: View information about IL-22 on UniProt.org
Clone: BL35175
Regulatory Status: RUO
Other Names: Interleukin-22, IL-TIF, IL-10-related T-cell-derived-inducible factor
Isotype: Rat IgG2a, κ