Biolegend, 517605, Biotin anti-human IL-2 Antibody, 50μg

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
50μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant human IL-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-2 protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate when paired with MQ1-17H12 (Cat. No. 500302). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection: The biotinylated Poly5176 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified MQ1-17H12 (Cat. No. 500302) antibody as the capture antibody.Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kits (Cat. No. 431807 & 431808) are specially developed and recommended.
Product Citations: Wang X, et al. 2022. Cancers (Basel). 14:. PubMed Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
RRID: AB_2564274 (BioLegend Cat. No. 517605)
Structure: Cytokine; 15.4 kD (Mammalian)
Bioactivity: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources: T cells
Cell Targets: T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors: High affinity heterotrimer of IL-2Ra/b/g, intermediate affinity homodimer IL-2Ra (CD25; p55; Tac) and heterodimer IL-2Rb (CD122)/g; g-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Taniguchi T, et al. 1993. Cell 73:5. 3. Nistico G. 1993. Prog. Neurobiol. 40:463. 4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
Regulation: Upregulated by NFAT; downregulated by TCF-8, CIF (colostrums inhibitory factor)
Gene ID: 3558
UniProt: View information about IL-2 on UniProt.org
Clone: Poly5176
Regulatory Status: RUO
Other Names: Interleukin-2, T cell growth factor (TCGF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I)
Isotype: Goat Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.