Biolegend, 523205, LEAF™ Purified anti-mouse LIF Antibody, 100μg

Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a type I transmembrane ligand-binding subunit, the LIF-specific subunit gp190 (LIFR), and the type I transmembrane signal transducing subunit gp130, shared with the other members of the IL-6 family. It especially signals through STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissues. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. LIF can be upregulated by proinflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
100μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant Mouse LIF
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: Neut - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by neutralization of proliferation inhibited by recombinant mouse LIF (Cat. No. 554002) at 0.5 ng/mL on M1 cells. ND50 range: 0.004 – 0.028 µg/mL. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2941596 (BioLegend Cat. No. 523205)
Structure: Monomer; mouse LIF is a 203 amino acid protein with a predicted molecular weight of approximately 22 kD.
Distribution: Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells
Function: Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemic cells
Interaction: Embryonic stem cells
Ligand/Receptor: LIFR
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Moreau JF, et al. 1988. Nature. 336:690-2. Tomida M, et al. 1993. FEBS Lett. 334:193-7. Metcalfe SM. 2011. Genes Immun. 12:157-68. Voyle RB, et al. 1999. Exp Cell Res. 249:199-211. Slaets H, et al. 2010. Trends Mol Med. 16:493-500.
Gene ID: 16878
UniProt: View information about LIF on UniProt.org
Clone: Poly5232
Regulatory Status: RUO
Other Names: Leukemia Inhibitory factor, HILDA, CDF, DIA, MLPLI
Isotype: Goat Polyclonal IgG
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.