Biolegend, 525402, Purified anti-human CD152 (CTLA-4) Antibody, 500μg

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein that plays an important regulatory role in the immune system. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. An identified novel transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only one of thirty apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human CTLA-4 recombinant protein ranging from 2500 to 39 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture: The purified A3.6B10.G1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated L3D10 antibody (Cat. No. 349911) as the detection antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407 & 437408) are specially developed and recommended.This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).
Product Citations: Ugbode CI, et al. 2017. J Neurochem. 429:142. PubMed
RRID: AB_2562988 (BioLegend Cat. No. 525401) AB_2563003 (BioLegend Cat. No. 525402)
Structure: Ig superfamily, 33 kD
Distribution: Activated T cells and B cells
Function: Negative regulator of T cell activation
Interaction: Antigen presenting cells, such as dendritic cells
Ligand/Receptor: B7-1 (CD80), B7-2 (CD86)
Cell Type: T cells, B cells, Dendritic cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego. 2. Kuiper H, et al. 1995. J. Immunol. 155:1776. 3. Lindsten T, et al. 1993. J. Immunol. 151:3489. 4. Morton P, et al. 1996. J. Immunol. 156:1047.
Gene ID: 1493
UniProt: View information about CD152 on UniProt.org
Clone: A3.6B10.G1
Regulatory Status: RUO
Other Names: Cytotoxic T-Lymphocyte Antigen 4 (CTLA4)
Isotype: Mouse IgG2a, κ