Biolegend, 532202, Purified anti-mouse NGAL (Lipocalin-2) Antibody, 500μg

NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. Mouse NGAL can be detected in the lung, spleen, uterus, vagina, and epididymis. It is a critical component of innate immunity due to its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising new markers for renal epithelial injury and cardiovascular disease (CVD).
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: In-house recombinant mouse NGAL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 1.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
Application Notes: The purified M047A10 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M0417A8 antibody as the detecting antibody. For testing mouse NGAL in cell culture supernatant, cell lysate, serum, plasma, urine, and other biological fluids, BioLegend's specially developed LEGEND MAX™ Kits are recommended.
RRID: AB_2650711 (BioLegend Cat. No. 532202)
Structure: 195 amino acid monomer with a predicted molecular weight of approximately 23 kD.
Distribution: Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.
Function: Apoptosis, innate immunity, renal development.
Interaction: Disulfide-linked with MMP9.
Ligand/Receptor: Apoptosis, innate immunity, renal development.
Cell Type: Epithelial cells, Macrophages, Neutrophils
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Iqbal N, et al. 2013. Expert Opin.Med. Diagn. 7:209. 2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663. 3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832. 4. Flo TH, et al. 2004. Nature 432:917. 5. Goetz DH, et al. 2002. Mol. Cell. 10:1033. 6. Leung L, et al. 2012. PLoS One 7:e46677. 7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.
Gene ID: 16819
UniProt: View information about NGAL on UniProt.org
Clone: M047A10
Regulatory Status: RUO
Other Names: Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Isotype: Rat IgG2a, κ